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Sukhramani, Prakash S.
- In-vitro Screening of Ficus racemosa for Anticancer Activity
Authors
1 JJT University, Vidyanagari, Jhunjhunu – Churu Road, Dist: Jhunjhunu, Rajasthan – 333001, IN
2 Veerayatan Institute of Pharmacy, Bhuj-Mandvi Road, Jakhania, Mandvi – 370460, Dist: Kutch, Gujarat, IN
3 Shree B.M Shah College of Pharmaceutical Education and Research, Modasa - 383315, Dist: Sabarkantha, Gujarat, IN
Source
Research Journal of Pharmacognosy and Phytochemistry, Vol 5, No 3 (2013), Pagination: 119-122Abstract
Ficus racemosa is a moderate sized avenue tree found throughout India. It is popular in indigenous system of medicine like Ayurveda, Siddha, Unani and Homoeopathy. Aim of the present study was undertaken with an objective to find out the anticancer activity of Ficus racemosa plant extracts using standard in-vitro MTT bioassay against various cancer cell lines (HL-60, HepG2, NCI-H23 and HEK-293T). With use of MTT dye, % cell viability and % inhibition of the hit compounds was evaluated within respective wavelengths prior with standard compounds. Data obtained from MTT bioassay screening revealed that methanolic extract of Ficus racemosa shown cytotoxic activity against HL-60 and HepG2 cell line with profound IC50 values and shown negligible toxicity against normal cell line (HEK- 293T).Keywords
Ficus racemosa Linn., MTT Bioassay, Anticancer Activity.
- Qualitative and Quantitative Standardization of Trikatu Churna
Authors
1 Veerayatan Institute of Pharmacy, Bhuj-Mandvi Road, Jakhania, Mandvi, Dist: Kutch – 370460, Gujarat, IN
2 Pioneer College of Pharmacy, Ajwa Road, Nr. N.H. 8, Sayajipura, Vadodara, Gujarat, IN
3 Department of Pharmaceutical Sciences, Saurashtra University, Rajkot-360 005, Gujarat, IN
Source
Research Journal of Pharmacognosy and Phytochemistry, Vol 3, No 4 (2011), Pagination: 154-157Abstract
The Ayurvedic system of medicine mentions many of churna formulation. One of them is Trikatu churna, nowadays most widely used in cold, cough, indigestion and gastric discomfort. Trikatu churna is finely powdered (# 80) mixture of Zingiber officinalis, Piper longum and Piper nigrum. The main object of this study was to check the quality of laboratory prepared Trikatu churna as well as to establish certain qualitative and quantitative parameters which may help in standardization of churna. The WHO guidelines were followed to evaluate laboratory preparation and individual drug component to gather the information regarding the quality. The standardization of churna was carried out by evaluating parameters like powder photomicroscopy, TLC study and preliminary phytochemical study. In addition to these, Trikatu churna and individual powder components were evaluated by physicochemical parameters like bulk density, % porosity, % compressibility, Hauser's ratio, flow property etc. These additional parameters were useful to establish certain quantitative standards for assessment of Trikatu churna. In microscopic study, we have identified the different cells and tissues which give clear identity of the content of Trikatu churna. The phytochemical screening test as well as simultaneous TLC study confirms presence of reported chemical constituents and presence of each individual drug in churna by comparing Rf values respectively.